ppten  (NSJ Bioreagents)

Journal: Antioxidants

Article Title: Intranasal Administration of KYCCSRK Peptide Rescues Brain Insulin Signaling Activation and Reduces Alzheimer’s Disease-like Neuropathology in a Mouse Model for Down Syndrome

doi: 10.3390/antiox12010111

Figure Lengend Snippet: Activation of insulin signaling following KYCCSRK intranasal administration in Ts2Cje mice. IR, IRS1, AKT, BVR-A, and PTEN protein levels and phosphorylation were evaluated in the frontal cortex of Eu and Ts2Cje (Ts-V) mice treated with vehicle (saline) and Ts2Cje (Ts-P) treated with KYCCSRK peptide (0.5 mM) for two weeks (n = 4/group). ( A ) Representative Western blot and total load images and densitometric evaluation of ( B , C ) IR protein levels and activation [evaluated as pIR Y1146/1150/1151 /IR ratio], ( D ) IRS1 protein levels, ( E ) IRS1 activation evaluated as pIRS1 Y612 /IRS1 ratio], ( F , G ) IRS1 inhibition [evaluated as pIRS1 S636 /IRS1 and pIRS1 S307 /IRS1 ratio], ( H , I ) AKT protein levels and activation (evaluated as pAKT S473 /AKT ratio), ( J ) BVR-A protein levels, and ( K , L ) PTEN protein levels and inhibition (evaluated as pPTEN S380/T382/383 /PTEN). All densitometric values were normalized per total protein load and are given as a percentage of Eu set as 100%. Data are shown as mean ± SEM. One-way ANOVA with Dunnett test: * p < 0.05, ** p < 0.01.

Article Snippet: pPTEN (S380/T382/383) , sc-101789 , SANTA CRUZ , 1:500.

Techniques: Activation Assay, Phospho-proteomics, Saline, Western Blot, Inhibition